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Analysis of optically active compounds using conventional chromatography with a circular dichroism detector
C Slijkhuis1, K D Hartog, C van Alphen
1KCF-Centre for Quality of Chemical-Pharmaceutical Products, RIVM-National Institute for Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands.
Journal of Pharmaceutical and Biomedical Analysis
|August 6, 2003
Summary
A new HPLC-CD method simplifies optically active compound analysis. It identifies enantiomers by peak sign, avoiding costly chiral methods for pharmaceuticals and supplements.
Area of Science:
- Analytical Chemistry
- Pharmacology
Background:
- Chiral analysis typically requires specialized chiral chromatography or capillary electrophoresis with expensive reagents or columns.
- Method selection is compound-dependent, limiting general applicability.
- Accurate identification of optically active compounds is crucial in pharmaceuticals and supplements.
Purpose of the Study:
- To present a simple, generally applicable method for analyzing optically active compounds.
- To demonstrate the utility of High-Performance Liquid Chromatography coupled with Circular Dichroism (HPLC-CD) detection.
- To enable enantiomer identification without prior separation.
Main Methods:
- Utilized a conventional High-Performance Liquid Chromatography (HPLC) column coupled to a Circular Dichroism (CD) detector.
- Employed the characteristic positive or negative peak signal generated by enantiomers.
- Analyzed standards, registered drugs, falsifications, and food supplements.
Main Results:
- HPLC-CD successfully identified enantiomers via distinct peak polarities.
- Racemates and optically inactive compounds did not produce a signal.
- Demonstrated applicability to diverse samples including dexamphetamine, 5-hydroxytryptophan, (-)-huperzine A, and interferon.
Conclusions:
- HPLC-CD offers a straightforward and broadly applicable approach for analyzing optically active compounds.
- This method bypasses the need for enantiomeric separation, reducing complexity and cost.
- Validated for identifying enantiomers in pharmaceutical products and food supplements.