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Updated: Aug 5, 2026

Identification of Plasmodesmal Localization Sequences in Proteins In Planta
Published on: August 15, 2017
Expression and localization of the linear DNA plasmid-encoded protein (RS224) in Rhizoctonia solani AG2-2
Atsushi Nagasaka1, Atsuko Sasaki, Toshiaki Sasaki
1Department of Environmental Biotechnology, Graduate School of Agriculture, Tohoku University, Sendai 981-8555, Japan.
Abstract:
Expression of the linear DNA plasmid-encoded protein (RS224) from the plant-pathogenic fungus Rhizoctonia solani isolate H-16, anastomosis group 2-2, and its localization were studied. Extracts from Escherichia coli cells expressing the open reading frame (ORF) of RS224 (RS224ORF in pRS224) contain a 92-kDa T7.Tag-RS224orf fusion protein. Antisera raised against the fusion protein obtained from E. coli cells cross-reacted with a 90-kDa protein in the mycelia. To analyze the subcellular localization of the 92-kDa protein, mycelia of R. solani were disrupted and fractionated. Antibodies against RS224 proteins specifically reacted to the mitochondrial fraction, suggesting that RS224 is localized in mitochondria.
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