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Updated: Sep 11, 2026

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
Published on: October 23, 2011
Development of specific fluorescent oligonucleotide probes for in situ identification of wine lactic acid bacteria
Lucía Blasco1, Sergi Ferrer, Isabel Pardo
1ENOLAB-Laboratori de Microbiologia Enològica, Departament de Microbiologia i Ecologia, Facultat de Ciències Biològiques, Universitat de València, Dr. Moliner 50, Burjassot, E-46100 Valencia, Spain.
Abstract:
A rapid method for the identification of lactic acid bacteria (LAB) from wine has been developed. This method is based on fluorescence in situ hybridisation (FISH), using fluorescent oligonucleotide probes, homologous to 16S rDNA of those species of LAB commonly found in wines. The protocol for the specific detection of these bacteria was established through the hybridisation of 36 reference strains. The specificity of the probes was evaluated by using pure cultures. Probes were used to identify species in different wines, making it evident that direct identification and quantification from natural samples without culturing is also possible. The results show that FISH is a promising technique for the rapid identification of LAB, allowing positive identification in a few hours (4-16 h).
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