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[Effects of growth regulator, full exposure and mist spray on the cutting of Eucommia ulmoides]
1Department of Biotechnology, Institute of Medicinal Plant Development, CAMS, PUMC, Beijing 100094, China. xjma@public.bta.net.cn
Objective:
To study the effects of growth regulator on the cutting method of Eucommia ulmoides seedlings.
Methods:
Treated with H2O (CK), NAA, 6-BA and NAA + 6-BA for 4 hours, respectively, cuttings of 4-year and 20-year trees were cultured for 45-60 days under the shed or full exposure and mist spray. The growth of the cuttings was observed periodically. The number of the roots, the root length, the callus and the death rate were investigated after harvest.
Results:
Compared with the cutting from 4-year-old tree, the cutting from 20-year-old tree developed better. The rate of stroking root in treated group was 14 times as much as CK group. Other agronomic traits such as the number of the roots, the root length, the callus, the number of the leaves and the death rate of the cuttings in treated group were all better than that of the cuttings in CK group. The cultural time in treated group reduced by 15 days than that of CK group.
Conclusions:
The present study shows that both full exposure mist spray and growth regulators have remarkable effects on cuttings of tree with different ages for rooting or forming callus.
