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Step-specific Sorting of Mouse Spermatids by Flow Cytometry
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Step-specific Sorting of Mouse Spermatids by Flow Cytometry

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Evaluation of spermatogenesis using flow cytometry and confocal microscopy

R Golan1, L M Lewin, Y Soffer

  • 1Department of Clinical Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel.

The Israel Medical Association Journal : IMAJ
|August 7, 2003
PubMed
Summary

No abstract available in PubMed .

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Spermatogenesis01:41

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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