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Dehydrated gelatin drops: a good method for fungi maintenance and preservation
A V Marangon1, T A Bertoni, E S Kioshima
1Division of Medical Mycology, Teaching and Research in Clinical Analyses Laboratory, Department of Clinical Analyses, State University of Maringá, Paraná, Brazil.
Abstract:
Some techniques have been proposed to maintain fungi culture collection. However, any choice must ensure the cultural stability and its phenotypic characteristics. This work proposes an adaptation of a preservation method considered by few literature reports: the dehydrated gelatin drops method (DGD). A total of 27 strains of fungi of clinical interest, including four dermatophyte fungi isolates, six filamentous non-dermatophyte fungi, five environment isolated filamentous fungi, six dimorphic fungi and six yeasts were maintained by this method for a seven year period at room temperature. After that time, the macro and micro characteristics of each fungus were studied, allowing the evaluation of the DGD method. In our experience, none of the strains maintained by DGD were found to be contaminated by bacteria or other fungi and no apparent changes were observed in morphology or macroscopic features.