Related Experiment Videos
Differentiation and malignant suppression induced by mouse erythroid differentiation and denucleation factor on mouse
Han Daishu1, Zhao Qing, Ge Yehua
1Department of Cell Biology, Institute of Basic Medical Sciences, CAMS & PUMC, Beijing 100005. daishu@pubic.bta.net.cn
Objective:
To investigate the roles of mouse erythroid differentiation and denucleation factor (MEDDF), a novel factor cloned in our laboratory recently, in erythroid terminal differentiation.
Methods:
Mouse erythroleukemia (MEL) cells were transfected with eukaryotic expression plasmid pcDNA-MEDDF. Then we investigated the changes on characteristics of cell growth by analyzing cells growth rate, mitotic index and colony-forming rate in semi-solid medium. The expressions of c-myc and beta-globin genes were analysed by semi-quantitative RT-PCR.
Results:
MEL cells transfected with pcDNA-MEDDF showed significant lower growth rate, mitotic index, and colony-forming rate in semi-solid medium (P<0.01). The percentage of benzidine-positive cells was 32.8% after transfection. The expression of beta-globin in cells transfected with pcDNA-MEDDF was 3.43 times higher than that of control (MEL transfected with blank vector, pcDNA3.1), and the expression of c-myc decreased by 66.3%.
Conclusions:
MEDDF can induce differentiation of MEL cell and suppress its malignancy.