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Expression of rhBMP-7 gene in transduced bone marrow derived stromal cells
Deyu Duan1, Jingyuan Du, Hong Wang
1Department of Orthopaedics, Union Hospital, Tongji Medical University, Wuhan 430022.
Abstract
Objective:
To explore the possibility of expression of exogenous gene in transduced bone marrow derived stromal cells (BMSCs).
Methods:
The marker gene, pbLacZ, was transferred into cultured BMSCs and the expression of transduced gene by X-gal staining was examined. Then plasmid pcDNA3-rhBMP7 was delivered to cultured BMSCs. Through immunohistochemical staining and RT-PCR assay, the expression of rhBMP7 gene was detected.
Results:
The exogenous gene could be expressed efficiently in transduced BMSCs.
Conclusion:
The present study provided a theoretical basis to gene therapy on the problems of bone and cartilage tissue.