PREPS and L-particles: a new approach to virus-like particle vaccines

Conventional virus-like particles are usually composed of a single structural protein which spontaneously assembles into particles. L-particles, a little-known type of virus-like particle, are produced as part of the natural infectious process of many, if not all, alpha-herpesviruses. L-particles lack the nucleocapsid present in the infectious virion but contain all of the virus envelope and tegument proteins. L-particles contain no virus DNA and are noninfectious, though they are biologically competent, since they are capable of delivering viral envelope and tegument proteins to cells. When cells are infected with herpes simplex virus Type 1 under conditions where viral DNA synthesis is blocked, previral DNA replication enveloped particles are produced. These are similar to L-particles, but differ slightly in protein composition. This article reviews the available data regarding these vaccine candidates and explores the wide-ranging potential applications, including vaccine candidates against infectious diseases and cancer, as well as a protein delivery vector.