Related Experiment Video
Updated: Jul 10, 2026

Using Coculture to Detect Chemically Mediated Interspecies Interactions
Published on: November 1, 2013
Development of a sensitive gene expression reporter system and an inducible promoter-repressor system for Clostridium
Laurence Girbal1, Isabelle Mortier-Barrière, Frédéric Raynaud
1Centre de Bioingénierie Gilbert Durand, Laboratoire de Biotechnologie-Bioprocédés, UMR CNRS 5504, UMR INRA 792, INSA, France. girbal@insa-tlse.fr
Abstract:
A sensitive gene expression reporter system was developed for Clostridium acetobutylicum ATCC 824 by using a customized gusA expression cassette. In discontinuous cultures, time course profiles of beta-glucuronidase specific activity reflected adequately in vivo dynamic up- and down-regulation of acidogenesis- and/or solventogenesis-associated promoter expression in C. acetobutylicum. Furthermore, a new inducible gene expression system was developed in C. acetobutylicum, based on the Staphylococcus xylosus xylose operon promoter-repressor regulatory system.
Related Concept Videos
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Reporter Genes
Commonly used reporter...
Inducible Operons: lac Operon

