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Comparative DNA sequence analysis of wheat and rice genomes
Mark E Sorrells1, Mauricio La Rota, Catherine E Bermudez-Kandianis
1Department of Plant Breeding, Cornell University, Ithaca, New York 14853, USA. mes12@cornell.edu
Abstract:
The use of DNA sequence-based comparative genomics for evolutionary studies and for transferring information from model species to crop species has revolutionized molecular genetics and crop improvement strategies. This study compared 4485 expressed sequence tags (ESTs) that were physically mapped in wheat chromosome bins, to the public rice genome sequence data from 2251 ordered BAC/PAC clones using BLAST. A rice genome view of homologous wheat genome locations based on comparative sequence analysis revealed numerous chromosomal rearrangements that will significantly complicate the use of rice as a model for cross-species transfer of information in nonconserved regions.