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[Establishment and preliminary application of screening methods for Y chromosome microdeletions in male infertility
Cong-yi Yu1, Guang-lun Zhuang, Can-quan Zhou
1Department of Obstetrics and Gynecology, Reproductive Medical Center, the Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510120 PR China. yucongyi01@sohu.com
Objective:
To develop a multiplex PCR protocol, which could be suitable for routine screening of microdeletions on the Y chromosome in azoospermic and oligozoospermic male infertility patients.
Methods:
Five multiplex sets were established. Eighty-seven azoospermic and oligozoospermic patients undergoing intracytoplasmic sperm injection (ICSI) in the in vitro fertilization (IVF) center and 30 azoospermic men undergoing testicular biopsy in the clinic of Urology Surgery were screened for microdeletions of Y chromosome.
Results:
A total of 19 (16.2%) cases of microdeletions were found in 117 azoospermic and oligozoospermic patients by screening of Y chromosome microdeletions. Of these, 11 cases (18.0%) were found in 61 oligozoospermic patients, and 8 cases (14.3%) were found in 56 azoospermic patients.
Conclusion:
The multiplex PCR protocol presented in this study is an easy-to-do and reliable method for detecting microdeletions on the Y chromosome. Routine screening of microdeletions on the Y chromosome for azoospermic and oligozoospermic patients is essential.
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