Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Recognition of DNA mismatch structures.

Shinya Hagihara1, Kazuhiko Nakatani, Isao Saito

  • 1Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 606-8501, Japan.

Nucleic Acids Research. Supplement (2001)
|August 9, 2003
PubMed
Summary

Researchers explored how naphthyridine-heterocycle hybrids bind to G-G mismatches. They discovered a two-step mechanism for zigzag intercalation, offering insights into DNA mismatch recognition.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Three-dimensional analysis of maxillary asymmetry in patients with mandibular prognathism.

Maxillofacial plastic and reconstructive surgery·2026
Same author

Plasma autoantibodies as novel risk markers for stroke: A nested case-control study of the JPHC study.

Clinica chimica acta; international journal of clinical chemistry·2026
Same author

Oxidative Stress Markers and the Risk of Incident Stroke and Ischemic Heart Disease: A Case-Cohort Study.

Journal of stroke·2026
Same author

Baseline leptin and longitudinal LDL-C changes in relation to sleep-disordered breathing: The Toon Health Study.

Journal of clinical lipidology·2026
Same author

Relationship between metabolic syndrome, metabolic syndrome-related factors, and all-cause mortality in the O City Cohort I survey: a 28-year follow-up study of rural Japanese residents.

Journal of rural medicine : JRM·2026
Same author

Associations of Serum Fatty Acids and the Eicosapentaenoic Acid/Arachidonic Acid Ratio with Hypertriglyceridemia in a Japanese Population: A Cross-Sectional Study.

Journal of atherosclerosis and thrombosis·2026

Area of Science:

  • Chemical Biology
  • Molecular Biology
  • Medicinal Chemistry

Background:

  • DNA mismatches, particularly G-G mismatches, are critical lesions in genetic material.
  • Understanding molecular recognition of DNA mismatches is vital for developing therapeutic agents.
  • 2-amino-1,8-naphthyridine derivatives are known to interact with nucleic acids.

Purpose of the Study:

  • To investigate the binding modes of naphthyridine-heterocycle hybrids to G-G mismatches.
  • To elucidate the structure-activity relationships governing this interaction.
  • To propose a general mechanism for the recognition of G-X mismatches by these compounds.

Main Methods:

  • Synthesis of novel naphthyridine-heterocycle hybrid molecules.
  • In vitro evaluation of binding affinity and mode to synthetic DNA duplexes containing G-G mismatches.
  • Structure-activity relationship (SAR) analysis.

Main Results:

  • A dimeric form of 2-amino-1,8-naphthyridine exhibits strong binding to G-G mismatches via a zigzag intercalation.
  • Naphthyridine-heterocycle hybrids were synthesized and their binding to G-G mismatches was characterized.
  • Structure-activity relationships suggest a general two-step scheme for zigzag binding to G-X mismatches (X = G, A, T).

Conclusions:

  • The study provides detailed insights into the molecular recognition of G-G mismatches by naphthyridine-heterocycle hybrids.
  • A generalizable two-step binding mechanism for G-X mismatches is proposed, based on structure-activity relationships.
  • These findings contribute to the rational design of novel DNA-interacting agents for potential therapeutic applications.

Related Experiment Videos