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Updated: Jul 29, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
Synthesis of 6-(2-thienyl)purine nucleoside derivatives toward the expansion of the genetic code
T Fujiwara1, H Sugiyama, I Hirao
1Yokoyama CytoLogic Project, ERATO, JST, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
Abstract:
Unnatural bases specifically pairing with pyridin-2-one, 2-amino-6-(2-thienyl) purine and 2-amino-6-(2-furanyl)purine, were newly designed to replace 2-amino-6-(N,N-dimethylamino)purine. It was expected that these novel purine analogues, as compared with 2-amino-6-(N,N-dimethylamino)purine, might reduce the interference in the stacking interactions with the neighboring bases in a duplex and improve the efficiency of the enzymatic incorporation of the nucleoside triphosphate of pyridin-2-one opposite these unnatural bases. The syntheses of these nucleoside derivatives and the DNA fragments were examined.
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