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Updated: Sep 20, 2026

The Lambda Select cII Mutation Detection System
Published on: April 26, 2018
DNA lesion and mutagenesis induced in phageM13mp2 by UVA, UVB and UVC irradiation
Y Izumizawa1, S J Yang, T Negishi
1Faculty of Pharmaceutical Sciences, Gene Research Center, Okayama University, Tsushima, Okayama 700-8530, Japan.
Abstract:
Sunlight is carcinogenic and mutagenic and its genotoxic effects are believed to be the result of UV light-induced lesions in DNA. These lesions include pyrimidine dimers and (6-4) photoproducts, but it is uncertain whether the pyrimidine modifications are the sole pre-mutagenic lesions induced by UV light. Previous studies indicate that some sunlight-induced mutations in the single-stranded DNA phage M13mp2 may not be caused by these photoproducts. In this work, purified single-stranded phage DNA was exposed to UVA, UVB and UVC and the induced mutations were analyzed. All 3 types of UV light increase the mutation frequency. The mutants were sequenced and the results suggest that UVA exposure may induce formation of a non-dipyrimidine lesion in DNA.
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