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Automated, solid-phase coupling of rhodamine dye acids to 5' amino oligonucleotides

R Vinayak1, H Tang

  • 1Applied Biosystems, 850, Lincoln Centre Drive, Foster City, CA 94404, USA.

Nucleic Acids Symposium Series
|August 9, 2003
PubMed

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Aryldiazonium Salts to Azo Dyes: Diazo Coupling01:11

Aryldiazonium Salts to Azo Dyes: Diazo Coupling

The reaction of weakly electrophilic aryldiazonium (also called arenediazonium) salts with highly activated aromatic compounds leads to the formation of products with an —N=N— link, called an azo linkage. This reaction, presented in Figure 1, is known as diazo coupling and occurs without the loss of the nitrogen atoms of the aryldiazonium salt. Highly activated aromatic compounds such as phenols or arylamines favor the diazo coupling reaction. The coupling generally occurs at the para position.