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[Preparation and identification of monoclonal antibodies against human apoptosis-related protein TFAR19]
1Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100083, China. yingyu_chen@yahoo.com
Objective:
To obtain mouse anti-human TFAR19 monoclonal antibodies for further study of the structure and function of the apoptosis-related protein TFAR19.
Methods:
BALB/c mice were immunized with recombinant human TFAR19, hybridoma cells were screened by cell fusion and subcloning approach. The monoclonal antibodies were identified by ELISA and Western blotting.
Results:
Three hybridoma cells (C1, C10, 2C12) stable in secreting anti-TFAR19 monoclonal antibodies were obtained. The monoclonal antibodies showed high specificity and high titer to TFAR19 with various affinity. All of them belong to IgG1 subclass.
Conclusions:
These monoclonal antibodies could bind specifically to TFAR19 protein.
Insights
Researchers developed mouse monoclonal antibodies targeting human TFAR19 protein. These antibodies are specific and suitable for studying the structure and function of TFAR19, an apoptosis-related protein.
Area of Science:
- Immunology
- Molecular Biology
- Cell Biology
Background:
- TFAR19 is an apoptosis-related protein.
- Understanding TFAR19's structure and function is crucial.
Purpose of the Study:
- To generate mouse anti-human TFAR19 monoclonal antibodies.
- To facilitate further research on TFAR19.
Main Methods:
- Immunization of BALB/c mice with recombinant human TFAR19.
- Hybridoma screening using cell fusion and subcloning.
- Antibody identification via ELISA and Western blotting.
Main Results:
- Three hybridoma cell lines (C1, C10, 2C12) stably secreting anti-TFAR19 antibodies were established.
- Obtained monoclonal antibodies demonstrated high specificity and titer for TFAR19.
- All antibodies were identified as IgG1 subclass.
Conclusions:
- The developed monoclonal antibodies specifically bind to the TFAR19 protein.
- These antibodies are valuable tools for TFAR19 research.
