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Published on: March 14, 2011
[Effect of 6A8 alpha-mannosidase expression on the proliferative response of human B cell line 3D5]
1Department of Immunology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China. zhaoft@hotmail.com
Objective:
To study the effect of 6A8 alpha-mannosidase expression on the proliferative response of human B cell 3D5.
Methods:
Recombination of adeno-associated virus vector(rAAV) mediated delivery of sense 6A8 DNA or antisense 6A8 DNA into 3D5 cells, and monoclonal antibody 6A8 alpha staining and Con A binding assay for determination of the change of 6A8 alpha-mannosidase expression, MTT assay for proliferation detection of 3D5 cells driven by Staphylococcus aureus crude cell suspension formalin-fixed (SAC), lower molecular weight B cell growth factor (LMW-BCGF), or rIL-6.
Results:
The expression of 6A8 alpha-mannosidase was enhanced in sense 6A8-transduced cells and reduced in antisense 6A8-transduced cells. In comparison with the wild type and the mock-transduced cells, the proliferative response of the sense 6A8-transduced cells to SAC stimulation was enhanced (P < 0.05). However, transduction with antisense 6A8 did not affect the response. In addition, transduction with either sense or antisense 6A8 had no effect on proliferation of 3D5 cells induced by LMW-BCGF or IL-6.
Conclusion:
The proliferative response to SAC stimulation was enhanced in the 3D5 cells with enhanced expression of 6A8 alpha-mannosidase. Either enhancement or reduction of 6A8 alpha-mannosidase expression had no effect on proliferation induced by LMW-BCGF or IL-6.
