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[Differentiation of human glioma cells BT-325 induced by sodium butyrate]
1Department of Biochemistry, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Objective:
To study the differentiation of human glioma cells BT-325 induced by sodium butyrate in vitro.
Methods:
BT-325 cells were treated with 1 mmol/L sodium butyrate. Flow cytometry was used to analyze the cell cycle. Cell differentiation was identified by flow cytometry and Western blot analysis.
Results:
After 6-8 days of sodium butyrate treatment, the differentiation characters could be observed distinctively, such as the reduced cell density, the increased cell size and the marked increase in cell process formation and cell-to-cell connection. At the same time, Western blot showed that the amount of glial fibrillary acidic protein was elevated after the sodium butyrate treatment.
Conclusion:
Human glioma cells BT-325 could be induced to differentiation by sodium butyrate.
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