Related Experiment Video
Updated: Sep 20, 2026

BRET-based G Protein Biosensors for Measuring G Protein-Coupled Receptor Activity in Live Cells
Published on: November 7, 2025
[G-protein modulates the inhibition of angiotensin II on BKCa in ECV304]
1Department of Physiology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Objective:
To investigate the effect of angiotensin II (A II) on maxi-conductance calcium-activated potassium channel(BKCa) activity in ECV304 cell membrane, and the possible involvement of G-protein.
Methods:
Cell-attached configuration of patch clamp technique was employed to record the current of BKCa in ECV304.
Results:
10(-7) mol/L angiotensin II inhibited the BKCa activity. Current amplitude and open probability were decreased, open time was shortened and close time was increased. G-protein activation could eliminate the inhibition of A II.
Conclusions:
A II significantly decreased the activity of BKCa in ECV304, which might depolarize the membrane, and the stability of membrane might change. Thus endothelial cell dysfunction might be induced. G-protein activation was involved in the regulation of the above A II inhibition process.
More Related Videos
08:46Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium
Published on: September 1, 2015
07:13Characterizing Modulators of Protease-Activated Receptors with a Calcium Mobilization Assay Using a Plate Reader
Published on: May 24, 2024
Related Concept Videos
GPCRs Regulate Adenylyl Cylase Activity
Two...
G-Protein Gated Ion Channels
Sensory organs,...
GPCR Desensitization
Activation and Inactivation of G Proteins
Antihypertensive Drugs: Angiotensin II Receptor Blockers
cAMP-dependent Protein Kinase Pathways