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Updated: Aug 1, 2026

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
Published on: February 8, 2016
[Stage-dependent expression of androgen binding protein mRNA in sertoli cell of rat testis]
1Department of Histology and Embryology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Objective:
To investigate the variation of rat androgen binding protein (ABP) mRNA in sertoli cell of rat testis during spermatogenesis.
Methods:
In situ hybridization was performed with digoxin-labeled rat ABP cDNA probe on frozen testis tissue sections from SD rats. The expression of ABP mRNA was quantified by using laser density scanning system.
Results:
It was shown that ABP mRNA levels were low from stage I to stage VI, increased to the peak level at the stages VII-VIII, and then declined rapidly at stages IX-XI. Thereafter, ABP mRNA levels increased again at stages XII-XIV, and then decreased to the lowest level at stages I-VI.
Conclusions:
ABP mRNA level was stage-dependent during the spermatogenic cycle.
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