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[Investigation of connective tissue growth factor's gene cloning]
1Department of Viral and Immunology, Institute of Medical Biology, CAMS, PUMC, Kunming 650118, China. glimbcam@km.ym.cn
Abstract
Objective:
The human connective tissue growth factor(CTGF), as the responsive product of immediate early gene, was cloned for the investigation of cellular primary gene responded and healing repairing.
Methods:
RT-PCR was performed with the mRNA of HUVE cell stimulated, the three clones of CTGF, via mutational modified, were ligated to a complete gene that identified with sequencing.
Results:
The sequencing indicated that this CTGF gene contains complete coding region and the 3'-noncoding region which is difference with the reported sequence.
Conclusion:
A new human CTGF gene was cloned.