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Updated: Sep 20, 2026
Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique
Published on: November 11, 2022
Effect of coxsackievirus b3 on ion channel currents in rat ventricular myocytes
1Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital of Shanghai Medical University, Shanghai 200032.
Objective:
To investigate the effects of coxsackievirus B3(CVB3) on ion channel currents in rat ventricular myocytes.
Methods:
Rat hearts were isolated with collagenase to acquire single ventricular myocytes, L-type voltage-dependent calcium channel (VDCC) current (I(Ca)), Na+ current (I(Na)), outward potassium current (I(out)), inwardly rectifying potassium current(I(KI)) were recorded using whole cell patch clamp techniques.
Results:
CVB3 infection increased I(Ca) and I(out), while decreased I(KI); but it had no obvious effect on I(Na).
Conclusion:
The effects of CVB3 an I(Ca), I(out), I(KI) may be one of the mechanisms of myocytes damage and the occurrence of abnormal electroactivities induced by CVB3 infection.
