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Published on: May 7, 2019
Expression of ICAM-1 and LFA-1 molecules in relation to renal allograft rejection in rats
1Department of General Surgery, The First Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041.
Objective:
The purpose of this study was to assess the renal graft expression of ICAM-1 (intercellular adhesion molecule-1) and LFA-1 (lymphocyte function-associated antigen-1) molecule with relation to graft rejection.
Methods:
Rat kidney transplantation was performed according to the procedure of Kamada with some modification. Experimental rats were divided into 5 groups. The survival time of recipient rats and function of grafts after renal transplantation were observed. The sections of renal graft were stained for monoclonal antibody ICAM-1 and LFA-1, and then quantification of ICAM-1 and LFA-1 expression was accomplished by computer image analysis.
Results:
ICAM-1 and LFA-1 increased significantly in the renal allograft rejection group as compared with the non-rejection groups (P<0.05).
Conclusion:
Both biopsy of renal graft and monitoring of ICAM-1 and LFA-1 are useful tools in diagnosing and treating acute rejection.
