Related Experiment Video
Updated: May 18, 2026
Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K
Published on: January 11, 2019
Identification of 100 KDa protein in sera of mice-treated with Cu(II) complex with superoxide dismutase-mimetic
OmAli Y El-Khawaga1, M M El-Naggar
1Biochemistry Division, Department of Chemistry, Faculty of Science, University of Mansoura, Mansoura, Egypt. elkhawaga70s@mans.edu.eg
Abstract:
The protein profile of sera isolated from mice pre-treated with Cu(II) complex of Girard T with superoxide dismutase (SOD)-mimetic activity was analyzed using SDS-PAGE. This complex was intraperitoneally administered (10 mg/Kg body weight) to Swiss albino mice. The resolved polypeptides showed a new sharp band at 100 KDa against which a polyclonal antibody was raised in rabbit. Sera of rabbit anti-100 KDa protein was used as a powerful probe for the detection of 100 KDa protein isolated from sera of treated mice. Western blot assays revealed a strong reactive polypeptide band at 100 KDa in sera of the mice, but no cross reaction was observed with sera of normal mice. The identification of purified polypeptide was confirmed by different characterization experiments.
More Related Videos
11:04Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
Published on: September 7, 2019
07:39Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line
Published on: April 13, 2022