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Updated: Aug 4, 2026
Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
Published on: February 1, 2014
Direct PCR detection of Escherichia coli O157:H7
K A Fode-Vaughan1, J S Maki, J A Benson
1Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI, USA.
Aims:
This paper reports a simple, rapid approach for the detection of Shiga toxin (Stx)-producing Escherichia coli (STEC).
Methods And Results:
Direct PCR (DPCR) obviates the need for the recovery of cells from the sample or DNA extraction prior to PCR. Primers specific for Stx-encoding genes stx1 and stx2 were used in DPCR for the detection of E. coli O157:H7 added to environmental water samples and milk.
Conclusions:
PCR reactions containing one cell yielded a DPCR product.
Significance And Impact Of The Study:
This should provide an improved method to assess contamination of environmental and other samples by STEC and other pathogens.
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