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Related Experiment Videos

Acidic alpha-D-mannosidase in phenotypically different leukemic lymphoid cells.

N A Ushakova1, R S Samojlova, M E Preobrazhenskaya

  • 1Institute of Biological and Medical Chemistry, Russian Academy of Medical Sciences, Moscow.

Biochemistry International
|December 1, 1992
PubMed
Summary
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Acid alpha-mannosidase activity varies in lymphoid cells from patients with lymphoproliferative disorders. This enzyme

Area of Science:

  • Biochemistry
  • Immunology
  • Hematology

Background:

  • Acid alpha-mannosidase is an enzyme with a role in cellular processes.
  • Lymphoproliferative disorders involve abnormal lymphocyte proliferation.
  • Understanding enzyme activity in these cells can aid diagnosis.

Purpose of the Study:

  • To investigate acid alpha-mannosidase activity in lymphoid cells from patients with lymphoproliferative disorders.
  • To correlate enzyme activity with specific cell immunophenotypes and disease types.
  • To explore the diagnostic potential of acid alpha-mannosidase activity in hematopoietic malignancies.

Main Methods:

  • Phenotypic characterization of lymphoid cells from peripheral blood, spleen, and lymph nodes.
  • Isolation of cells based on immunophenotype.

Related Experiment Videos

  • Assay of acid alpha-mannosidase enzyme activity in isolated cell populations.

    • Main Results:

    • Significant variations in acid alpha-mannosidase activity were observed across different lymphoid cell types.
    • Lowest activity was found in early B cells from B-cell chronic lymphocytic leukemia (B-CLL) patients.
    • Highest activity was detected in activated, CD11c-expressing B cells from B-cell non-Hodgkin lymphoma (B-NHL) and hairy cell leukemia (HCL) patients.
    • Differences in enzyme activity were noted between peripheral blood and spleen cells in B-NHL patients with spleen involvement.

    Conclusions:

    • Acid alpha-mannosidase activity is a potential biomarker for classifying and diagnosing lymphoproliferative disorders.
    • Enzyme activity levels correlate with specific immunophenotypes and disease subtypes.
    • These findings may contribute to improved staging and management strategies for hematopoietic malignancies.