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Updated: Jul 29, 2026
A Murine Closed-chest Model of Myocardial Ischemia and Reperfusion
Published on: July 17, 2012
[Experiment study of c-fos expression on myocardial acute ischemia/reperfusion injury in rats]
1Shenzhen Criminal Science & Technology Institute, Shenzhen 518008, China.
Objective:
To investigate the changes of c-fos mRNA induced by myocardial ischemia/reperfusion(I/R) during acute period.
Methods:
The model of I/R was established, and the rats was divided normal control, ischmia and I/R groups. Insitu hybridization (ISH) and computerized image analysis method was used to observe alteration of c-fos mRNA in cardiac myocytes.
Results:
After 10 min ischemia and 30 min reperfusion, the area of reperfusion showed a part of cardiac myocytes weak staining, and get to peak at I-60 min/R-30 min. The myocardium in normal control and ischemia groups less than 60 min showed negative staining. No changes were found in all groups by HE staining.
Conclusion:
c-fos mRNA detection may become an important method for diagnosis of I/R.
