Related Experiment Videos
[Cloning, expression and purification of neural specific HuD cDNA]
Jian-hua Chen1, Xiu-qin Liu, Yu-pu Guo
1Department of Neurology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Abstract
Objective:
To prokaryoticly express and purify HuD protein and its RNA recognition motifs.
Methods:
HuD protein was prokaryoticly expressed and purified by molecular cloning technology. Its biologic activity was testified by Western Blot.
Results:
Purified HuD protein and its RNA recognized motifs were observed.
Conclusions:
The result might aid for basic research and clinical application.