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[Cloning cDNA encoding for GAGA-like element binding proteins in human Jurkat cells]
Zhi-cheng Mo1, Yu Lu, Chao Gao
1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Summary
Researchers screened for GAGA-like element binding proteins in human Jurkat cells using a yeast one-hybrid system. Six candidate proteins were identified for further study.
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Context:
- The GAGA-like element binding proteins play crucial roles in gene regulation within human cells.
- HTLV-1 transformed Jurkat cells provide a relevant model system for exploring these proteins.
Purpose:
- To identify and characterize GAGA-like element binding proteins in human Jurkat cells.
- To screen a cDNA fusion library derived from HTLV-1 transformed Jurkat cells.
Summary:
- A yeast one-hybrid system was employed to screen for proteins that bind to GAGA-like elements.
- Total RNA from Jurkat cells was reverse transcribed, labeled, and used as a cDNA probe.
- Nine positive clones were identified, with six showing positive hybridization to the cDNA probe.
Impact:
- This study successfully identified six candidate clones encoding GAGA-like element binding proteins.
- These findings provide a foundation for further investigation into the function and significance of these proteins in human cells.