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[Establishment and application of a RT-PCR system for quantifying mRNA of 4 hsp genes in human cells]
Hong-fan Li1, Xing-rong Liu, Feng-yan Heng
1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Objective:
To establish a RT-PCR system for detecting mRNA expression level of 4 hsp genes in human cells.
Methods:
RT-PCR system was established with gene cloning, gene recombination, in vitro transcription and RT-PCR techniques; The detection of expression level of 4 hsp genes in SW13 cell was carried out with this system.
Results:
In SW13 cell, hsp70 and hsp90 alpha were typical heat shock induced genes, while hsp60 and hsp90 beta were efficiently expressed and further induced by heat-shock to various extent.
Conclusions:
In our hands novel RT-PCR system can be used to detect mRNA expression level of 4 human hsp genes.
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