[Genotyping of HLA-DRB1 by PCR-SSP in Yunnan Lahu ethnic groups]
Kun-xue Hong1, Qin He, Xi-yun San
1Department of Genetics, Institute of Medical Biology, CAMS, PUMC, Kunming 650118, China.
Objective:
To investigate polymorphism of HLA-DRB1 in Chinese Lahu population in Yunnan.
Methods:
Polymerase chain reaction-sequence specific primers (PCR-SSP) were used to determine HLA-DRB1 genotypes of 110 unrelated healthy Lahu minority people of Yunnan Province.
Results:
Sixteen alleles of DRB1 were detected in this study. The results of test showed that the genotype distributions observed were corresponded with the Hardy-Weinberg equilibrium.
Conclusion:
This study has obtained a more comprehensive and accurate data set of the normal allele frequencies of HLA-DRB1 in Chinese Lahu population in Yunnan Province, which may be of significance in the studies on population genetics and disease association.
