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Updated: Sep 20, 2026
Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells
Published on: December 3, 2015
[Construction of recombinant human CD59 using ICAM-2 promoter for endothelial-specific expression in
Xu-dong Yao1, Teng-xiang Ma, Zhi-hong Wu
1Tianjin Institute of Urology Surgery, Tianjin Medical University, Tianjin 300211, China. yaoxu7962_cn@sina.com
Objective:
To construct a recombinant human CD59 gene containing intercellular adhesion molecule-2 promoter for high level endothelial-specific expression in xenotransplantation.
Methods:
ICAM-2 promotor fragment and CD59-intron 1 fragment were produced by PCR from the human blood genome, and then clone these fragments into a pcDNA3-CD59 eukaryotic expression vector which was followed by digestion with the specific restricted endonuclease (for example: EcoRI, Hind III). The ICAM-2 promoter and CD59-intron 1 fragments were identified by PCR, and sequencing. The recombinant was then transfected into pig aorta endothelial cells with Lipofection, and the expression was measured by flow cytometer.
Results:
Products of the sequences measured were in accord with the frames of the gene bank. The expression of the protein of this recombinant was positive.
Conclusion:
The CD59 recombinant gene is constructed successfully, providing a basis for transgenic research.
