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[Expression of rat protamine gene in MEL cells]
Yu Chen1, Ren-ren Fei, Xiao-fang Wu
1Institute of Materia Medica, CAMS, PUMC, Beijing 100050, China.
Abstract
Objective:
To investigate the expression of rat protamine (RP) gene in MEL cells and the effect on cell growth.
Methods:
Eukaryotic expression plasmid pCR-3.1-RP was constructed and transfected into MEL cells. The changes of cell growth rate, mitotic index and colony-forming rate in semi-solid medium were investigated.
Results:
Transfected MEL cells showed lower growth rate, mitotic index and colony-forming rate. Volumes of cells were reduced and reduction of RNA transcription was observed.
Conclusion:
These results suggest that expression of RP in MEL cell may inhibit the cell growth and proliferation.