[Gene clone and its characteristics on band 7-like protein in Plasmodium falciparum FCC1/HN]
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Published on: November 22, 2024
Ling Zhang1, Lian-hui Zhang, Hai-yi Wang
1Etiology Department, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Insights
Researchers identified and cloned the Plasmodium falciparum stomatin (PfStom) gene, revealing its expression in the trophozoite stage. This membrane-related protein belongs to the band 7 family, offering a foundation for further malaria research.
Area of Science:
- Molecular Biology
- Parasitology
- Genomics
Context:
- The Plasmodium falciparum stomatin (PfStom) gene encodes a protein within the band 7 family.
- Understanding the function and expression of novel Plasmodium falciparum genes is crucial for malaria parasite research.
Purpose:
- To identify and clone the pfstom gene from Plasmodium falciparum.
- To characterize the PfStom protein's family classification and expression patterns.
- To lay the groundwork for future functional studies of PfStom.
Summary:
- The pfstom gene, 1,125 bp in length, encodes 374 amino acids. The C-terminal fragment shows homology to stomatin-like proteins of the band 7 family.
- Phylogenetic analysis confirmed homology to stomatin.
- Western blot analysis demonstrated stage-specific expression of PfStom in the trophozoite stage of the malaria parasite within erythrocytes, with no expression observed in the ring stage.
Impact:
- PfStom is identified as a membrane-related protein belonging to the band 7 family.
- The study establishes the stage-specific expression of PfStom during the erythrocytic cycle of Plasmodium falciparum.
- These findings provide a critical foundation for further investigations into the role of PfStom in malaria pathogenesis.
Objective:
To identify and clone the gene named pfstom gene which encoding the protein belonging to band 7 family and to do primary research on its function.
Methods:
Based on the finished data in international public malaria database, coding sequence of pfstom cDNA was obtained by RT-PCR from FCC1/HN. Its phylogenetic profiles and the homogeny were analyzed by some softwares. After Prokaryotic expression, C terminal of Pfstom protein was expressed by Pet30a system. Recombinant Pfstom protein was used to immol/Lunize rabbit and then serum was harvested and the IgG was purified for Western blot.
Results:
The coding sequence of pfstom is 1,125 bp which encoding 374 amino acids with C-terminal fragment being homogenous to stomatin-like protein which belongs to band 7 family. Phylogenetic profiles analysis revealed its homogeny to stomatin. Western blot showed its stage-specific expression in trophozoite.
Conclusion:
Pfstom belongs to band-7 family. It was expressed specifically in trophozoite in erythrocyte stage of plasmodium falciparum. It was not expressed in ring stage. And it is membrane-related protein. All these results provided the foundation for further research on pfstom.
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