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[The transcriptional regulation study on human delta globin gene with CAAT box C-->T point mutation in its promoter]
Jie Yao1, Song-sen Chen, Ke-gong Yang
1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Objective:
To study the transcriptional regulation of human delta globin gene with C-->T point mutation at -64 in its promoter.
Methods:
Human delta globin genes including wild CAAT box and mutant CAAT box (-64C-->T) were separately cloned into eukaryotic expression vector pcDNA3.1 (-)/Myc-His A which was cut out the strong promoter CMV, transfected MEL cells, and induced by DMSO to express. The transcriptional regulation of human delta globin gene was analysed using semi-quantitative RT-PCR.
Results:
The expression level of human delta globin gene with mutant CAAT box was 2.2-fold as high as that with wild CAAT box.
Conclusion:
The defective CAAT box of human delta globin gene promoter region may be one of the major reasons for its low expression level.
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