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Updated: Sep 1, 2026

Preparation of Mitochondria from Ovarian Cancer Tissues and Control Ovarian Tissues for Quantitative Proteomics Analysis
Published on: November 18, 2019
[Mutations in the D-loop region of mitochondrial DNA in ovarian tumors]
Hong-hui Shi1, Liu Vincent, Ngan Hextan
1Department of Obstetrics and Gynecology, PUMC Hospital, CAMS, PUMC, Beijing 100730, China.
Objective:
To investigate mutations in the D-loop region of mitochondrial DNA in ovarian tumors.
Methods:
The D-loop region of 25 epithelial ovarian tumors together with the adjacent normal tissues were amplified by PCR and sequenced.
Results:
Among the 25 ovarian tumors, 26 mutations were identified with the mutation rate of 32%. 19 mutations were detected in two cases of borderline carcinoma which was a special type of epithelial ovarian carcinoma. There were 6 microsatellite instabilities among the mutations and 11 new polymorphisms which were not reported previously in the GenBank.
Conclusions:
The D-loop region of mitochondrial DNA is a highly polymorphoric and mutable region and the mutation rate is relatively high in patients with ovarian tumors.
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