[Expression of neurotrophin-4(NT-4) gene in baculovirus expression vector system]
1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China.
Objective:
We selected baculovirus expression vector system to express human NT-4 with biological activity.
Methods:
The hNT-4 mature peptide-coding sequence is amplified by polymerase chain reaction (PCR), ligated to baculovirus expression vector PacGP67B, and expressed in the insert sf9 cell line.
Results:
The protein molecular weight of the expressed hNT-4 was about 15,000 by SDS-PAGE, and the hNT-4 antibody could react with this protein in the infected supernatant and total cell by western-blot. The activity of hNT-4 determined by PC12 cell line was definite.
Conclusions:
The results may aid for studying the effect of the hNT-4 on basic medical research and clinical application.
