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[Eukaryotic expression and in vitro tumor cell apoptosis-induced effect of human TRAIL]
1Molecular Immunology Laboratory, Peking University, Medical Immunology Key Laboratory of Health Ministry of China, Peking University Center for Human Disease Genomics, Beijing 100083, China. xu.shi@mailcity.com
Objective:
To explore the tumor cell apoptosis induced by the effect in vitro of eukaryotic expression of full-length TRAIL cDNA and its extracellular region.
Methods:
The eukaryotic expression vectors for both forms of the cDNA acquired from the fetal heart cDNA library were constructed. After gene transfecting, the stable expression cell lines were obtained by G418 screening.
Results:
The supernatants from tansfectants could induce apoptosis of different tumor cell lines in vitro, and an enhanced effect was observed by adding TFAR19 (TF-1 cell apoptosis-related protein 19), a novel apoptosis gene product discovered in our laboratory.
Conclusion:
Eukaryotic expression products of TRAIL can induce apoptosis of the tumor cells, and TFAR19 could enhance the effect on apoptosis of tumor cells.