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Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic
Published on: September 4, 2012
L-selectin and beta2-integrin expression on circulating bovine polymorphonuclear leukocytes during endotoxin mastitis
A Diez-Fraile1, E Meyer, L Duchateau
1Department of Physiology, Biochemistry and Biometrics, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Journal of Dairy Science
|August 9, 2003
Summary
Intramammary lipopolysaccharide (LPS) administration in cows alters adhesion molecule expression on neutrophils. This impacts inflammatory responses and neutrophil behavior during bovine mastitis.
Area of Science:
- Veterinary Immunology
- Bovine Mastitis Research
- Inflammatory Response Mechanisms
Background:
- Bovine mastitis, particularly coliform mastitis, involves complex inflammatory pathways.
- Adhesion molecules on neutrophils play a critical role in their migration and function during infection.
- Understanding endotoxin's impact on these molecules is key to managing mastitis.
Purpose of the Study:
- To investigate the in vivo effect of intramammary endotoxin (lipopolysaccharide, LPS) on bovine neutrophils.
- To analyze the expression of L-selectin (CD62L) and beta2-integrin subunits (CD11b, CD18) on circulating neutrophils.
- To correlate changes in adhesion molecule expression with clinical parameters of inflammation.
Main Methods:
- Intramammary infusion of Escherichia coli LPS into early lactating cows.
- Flow cytometric analysis of cell surface adhesion molecule expression on peripheral blood neutrophils.
- Measurement of clinical parameters: rectal temperature, blood cortisol, somatic cell count (SCC), and leukocyte counts.
Main Results:
- LPS infusion decreased CD62L expression and increased CD11b and CD18 density on neutrophils.
- Changes in adhesion molecule expression correlated with decreased circulating neutrophils, fever, elevated cortisol, and increased SCC.
- No direct correlation found between CD11b and CD18 expression kinetics.
Conclusions:
- Intramammary LPS administration significantly modulates adhesion receptor expression on bovine neutrophils.
- Endotoxin plays a key role in the inflammatory cascade during bovine mastitis.
- CD18 variation during endotoxemia is not solely driven by CD11b upregulation, differing from clinical mastitis observations.
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