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Updated: Sep 20, 2026
Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium
Published on: July 14, 2020
MfERG waveform characteristics in the RS1h mouse model featuring a 'negative' ERG
Mathias W Seeliger1, Bernhard H F Weber, Dorothea Besch
1Retinal Electrodiagnostics Research Group, University Eye Hospital Department II, Schleichstr. 12-16, D-72076 Tübingen, Germany. see@uni-tuebingen.de
Abstract:
Several retinal disorders lead to a relatively greater attenuation of the b-wave compared to the a-wave of the electroretinogram (ERG), a constellation called 'negative' ERG. To determine the waveform characteristics of multifocal ERGs (mfERGs) and their dependence on recording parameters in such a case, we studied the Rs1h(-/Y) mouse, the model for x-linked juvenile retinoschisis. mfERGs were recorded with a VERIS 4 system connected to a piggyback stimulator prototype that added the stimulus to the optical pathway of a HRA scanning-laser ophthalmoscope (SLO) by means of a wavelength-sensitive mirror. Real-time fundus visualization was achieved with the infrared laser of the SLO (835 nm). High-pass filter settings and the time interval used by the 'artefact removal' feature were varied to study their influence on the waveform. The mfERG in the Rs1h(-/Y) mouse had a 'negative' shape. However, the high-pass filter setting had to be lowered from the usual 10 Hz down to about 2 Hz in order to obtain that result, otherwise the negative shape was lost and mainly a positive peak remained. Similarly, a short time interval used by the 'artefact removal' feature also removed the negative shape. The Rs1h(-/Y) mouse was found to be a valuable model of diseases with a 'negative' waveform shape also in mfERG. Our results underline the importance of a lower high-pass filter cutoff frequency when recording mfERGs in such disorders. In addition, if the 'artefact removal' feature is used, it should be verified that it doesn't distort the waveform shape.
Insights
The Rs1h(-/Y) mouse model exhibits a
Area of Science:
- Ophthalmology
- Neuroscience
- Genetics
Background:
- Retinal disorders can cause a 'negative' electroretinogram (ERG) waveform, characterized by reduced b-wave amplitude relative to the a-wave.
- X-linked juvenile retinoschisis is a condition associated with 'negative' ERG findings.
Purpose of the Study:
- To characterize multifocal electroretinogram (mfERG) waveforms in the Rs1h(-/Y) mouse model of x-linked juvenile retinoschisis.
- To investigate the influence of recording parameters, specifically high-pass filter settings and artifact removal, on mfERG waveform shape in this model.
Main Methods:
- mfERGs were recorded using a VERIS 4 system integrated with a scanning-laser ophthalmoscope (SLO) for real-time fundus visualization.
- High-pass filter settings were adjusted (from 10 Hz down to 2 Hz) and the artifact removal feature's time interval was varied to assess their impact on waveform morphology.
Main Results:
- The Rs1h(-/Y) mouse model demonstrated a 'negative' mfERG waveform when a lower high-pass filter (around 2 Hz) was employed.
- Standard high-pass filter settings (10 Hz) or short artifact removal intervals obscured the 'negative' waveform, resulting in a predominantly positive peak.
Conclusions:
- The Rs1h(-/Y) mouse is a suitable model for studying retinal diseases with 'negative' ERG waveforms.
- Accurate recording of 'negative' mfERG waveforms in such conditions requires lower high-pass filter cutoff frequencies and careful use of artifact removal settings to prevent waveform distortion.
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