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Determination of urinary hippuric acid by micellar electrokinetic capillary chromatography
Cecilia Zuppi1, Diana Valeria Rossetti, Alberto Vitali
1Institute of Biochemistry and Clinical Biochemistry, Catholic University of Rome, Rome, Italy. czuppi@rm.unicatt.it
Abstract:
We propose a method for the simultaneous determination of hippuric acid (HA) and creatinine based on capillary micellar electrokinetic chromatography. Experimental conditions were 20 mM sodium phosphate, pH 7.20, 25 mM sodium dodecyl sulfate, 5% (v/v) acetonitrile. Electropherograms evidenced HA and creatinine peaks in less than 12 min. The method showed good linearity for both analytes and satisfactory within-day precision. The present method, which is accurate, sensitive, rapid and simple, may be applied to single-spot urine samples.