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Related Experiment Videos

Identification of 5-fluorouracil-inducible target genes using cDNA microarray profiling.

Pamela J Maxwell1, Daniel B Longley, Tariq Latif

  • 1Department of Oncology, Cancer Research Centre, Queen's University Belfast, University Floor, Belfast City Hospital, Lisburn Road, Belfast BT9 7AB, Northern Ireland.

Cancer Research
|August 9, 2003
PubMed
Summary

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Researchers identified key genes, including SSAT and MAT-8, activated by 5-Fluorouracil (5-FU) chemotherapy. These genes, regulated by p53, may indicate tumor response and resistance to cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • 5-Fluorouracil (5-FU) is a cornerstone chemotherapy agent for various cancers.
  • Understanding downstream mediators of 5-FU response is crucial for optimizing cancer therapy.
  • Identifying novel genes involved in chemoresistance can lead to better predictive biomarkers.

Purpose of the Study:

  • To identify novel genes transcriptionally activated by 5-FU in breast cancer cells.
  • To investigate the role of p53 in regulating 5-FU-induced gene expression.
  • To explore the potential of identified genes as biomarkers for 5-FU resistance.

Main Methods:

  • DNA microarray analysis of MCF-7 breast cancer cells treated with 5-FU.
  • Northern blot validation for highly up-regulated genes.

Related Experiment Videos

  • Analysis of gene expression in 5-FU-resistant colorectal cancer cell lines.
  • Investigation of p53's role through gene inactivation experiments.

    • Main Results:

    • 619 out of 2400 analyzed genes were up-regulated by 5-FU.
    • Key up-regulated genes identified: SSAT, annexin II, thymosin-beta-10, chaperonin-10, and MAT-8.
    • p53 plays a significant regulatory role in the 5-FU-induced expression of these genes.
    • Elevated basal expression of these genes was observed in a 5-FU-resistant cell line, suggesting biomarker potential.

    Conclusions:

    • DNA microarrays are effective for discovering novel genes involved in chemotherapy response.
    • SSAT, annexin II, thymosin-beta-10, chaperonin-10, and MAT-8 are potential mediators of 5-FU action.
    • p53 is a key regulator of 5-FU-induced gene expression.
    • These genes may serve as valuable biomarkers for predicting 5-FU resistance in colorectal cancer.