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Related Experiment Videos

Long-term continuous evolution of acetate resistant Acetobacter aceti.

Peter Steiner1, Uwe Sauer

  • 1Institute of Biotechnology, ETH Zürich, CH-8093 Zürich, Switzerland.

Biotechnology and Bioengineering
|August 12, 2003
PubMed
Summary

Acetic acid bacteria evolved high acetate resistance through genetic adaptation. Evolved bacteria maintained lower intracellular acetate levels, suggesting a key mechanism for overcoming toxicity.

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Area of Science:

  • Microbiology
  • Environmental Science
  • Biochemistry

Background:

  • Cytotoxic acetate is prevalent in various environments.
  • Acetic acid bacteria exhibit remarkable acetate resistance, crucial for industrial applications.
  • Understanding acetate resistance mechanisms is vital for microbial biotechnology.

Purpose of the Study:

  • To investigate the genetic basis of high-level acetate resistance in Acetobacter aceti.
  • To compare the adaptive capabilities of acetate-resistant and acetate-sensitive bacteria under selective pressure.
  • To elucidate the role of intracellular acetate accumulation in acetate toxicity.

Main Methods:

  • Long-term continuous cultures of Acetobacter aceti and Escherichia coli with increasing acetate concentrations.

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  • Monitoring bacterial growth and resistance evolution over 240 generations.
  • Quantifying intracellular acetate concentrations in wild-type and evolved strains.

    • Main Results:

    • Acetobacter aceti evolved significant acetate resistance, growing at over 50 g/L within 240 generations.
    • Acetate-sensitive Escherichia coli showed no significant resistance acquisition.
    • Evolved Acetobacter aceti maintained significantly lower intracellular acetate levels compared to wild-type and E. coli.

    Conclusions:

    • Acetobacter aceti possesses a genetically determined mechanism for rapid acetate resistance evolution.
    • Reduced cytoplasmatic anion accumulation is a critical factor in overcoming acetate toxicity.
    • This study provides insights into microbial adaptation to environmental stressors and biotechnological potential.