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[Screening of hypercoagulability-related genes in aorta of hypertriglyceridemic rats]
Yuhua Zhao1, Bingwen Liu, Huai Bai
1Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Objective:
The aim of this study was to screen hypercoagulability-related genes in aorta of hypertriglyceridemic rats.
Methods:
Suppression subtractive hybridization (SSH) was used to construct hypercoagulable rat cDNA subtractive library. Semi-quantitative RT-PCR was applied to identify differentially expressed genes.
Results:
Hypercoagulable rat cDNA subtractive library had been successfully constructed. Sequence analysis and homology searching of 20 clones showed that these clones represent 19 genes: 8 are known genes newly found to have a relationship to hypercoagulability and 11 are novel genes (ESTs) that had been deposited to GenBank. Three ESTs (HC002, HC057 and HC067) were confirmed to be over-expressed genes by semi-quantitative RT-PCR.
Conclusion:
The constructed subtractive library is highly efficient and has laid a foundation for screening and cloning hypercoagulability-related novel genes of rat, HC002, HC057 and HC067 are probably related to hypercoagulability induced by hypertriglyceridemia.