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[Effect of Fos expression induced by spinal nitric oxide on the nociception of formalin in rats]
Xuemei Lin1, Quanyun Wang, Lin Zhu
1Department of Anesthesiology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To assess the effect of s.c. injected formalin and clarify the possible mechanism of nociception in SD rats.
Methods:
Sixty-four healthy male rats were randomly distributed into the control groups (group NS, group formalin) and test groups (group LaF, group LnF) with correponding numbers. Group NS served as normal control. Group formalin was treated with 5% formalin 100 microliters, s.c. Groups LaF and LnF were treated respectively with L-Arg 4.7 mumol ith and L-NAME 370 nmol ith before receiving 5% formalin 100 microliters, s.c. like group formalin. 1/2 h and 0-1 h after formalin treatment, spinal cord segments were removed for expression of NOS, and behavior responses were observed.
Results:
The flinching and licking time of group formalin was longer than that of group NS, and NOS expression of group formalin was stronger than that of group NS. Pretreatment with L-Arg potentiated formalin-induced response, but pretreatment with L-NAME reduced the response.
Conclusions:
Spinal nitric oxide release might be one of the nociception mechanisms in the formalin test.
