Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Blood storage for forensic hemoglobin analysis using CO-oximeter.

Naoya Watanabe1, Koichi Terazawa, Mitsuo Sakaihara

  • 1Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan.

[Hokkaido Igaku Zasshi] the Hokkaido Journal of Medical Science
|August 13, 2003
PubMed
Summary

Proper blood sample storage is crucial for forensic CO-oximeter analysis. Refrigeration at 4°C without air contact preserves hemoglobin derivatives for up to two weeks, while frozen storage is best for total hemoglobin.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

"Longitudinal Ultrasonographic Evaluation of Hemodynamic Changes at End-to-Side Anastomoses to Major Arteries in Limb Free Flap Surgery".

Plastic and reconstructive surgery·2026
Same author

The utility of locking plate fixation combined with intraosseous wiring for an intra-articular depressed fracture of the middle phalangeal base: A case report.

Trauma case reports·2026
Same author

Corrigendum to "Prophylactic versus reactive leech therapy for venous congestion after fingertip replantation: A retrospective comparative study and literature review" [JPRAS Open 46 (2025) 216-229].

JPRAS open·2026
Same author

Prophylactic versus reactive leech therapy for venous congestion after fingertip replantation: A retrospective comparative study and literature review.

JPRAS open·2025
Same author

A Novel Ultrasound-guided Forearm Continuous Peripheral Nerve Block Technique Using Bidirectional Hydrodissection.

Plastic and reconstructive surgery. Global open·2025
Same author

Free Flap Surgery for Elbow Soft Tissue Reconstruction Using the Brachial Artery as Recipient Vessel: Evaluation of MPETS Cases and Comparative Literature Review.

Medicina (Kaunas, Lithuania)·2025

Area of Science:

  • Forensic Science
  • Biochemistry
  • Analytical Chemistry

Background:

  • Accurate forensic analysis of blood samples relies on preserving hemoglobin derivative integrity.
  • CO-oximetry is essential for post-mortem and re-examination of blood samples.

Purpose of the Study:

  • To determine optimal storage conditions (temperature and air contact) for preserving hemoglobin derivatives in blood samples for CO-oximetry.

Main Methods:

  • Blood samples were stored under various conditions: different temperatures (37°C, room temp, 4°C, -20°C) and with or without air contact.
  • Hemoglobin derivatives (O2Hb, MetHb, COHb, HHb, THb) were analyzed using CO-oximetry over time (up to 6 weeks, and 6 months for COHb).

Main Results:

  • Storage at 4°C without air contact preserved O2Hb, MetHb, COHb, and HHb for up to 2 weeks.

Related Experiment Videos

  • Carboxyhemoglobin (COHb) remained stable for at least 6 months at 4°C without air contact.
  • Frozen storage (-20°C) was optimal for preserving total hemoglobin (THb) for 4-6 weeks.

    • Conclusions:

    • Optimal blood sample storage for CO-oximetry depends on the specific hemoglobin derivative and desired analysis period.
    • Refrigeration at 4°C without air contact is recommended for short-term preservation of most hemoglobin derivatives.
    • Long-term COHb preservation is achievable at 4°C, while THb requires frozen storage.