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Updated: Sep 20, 2026

Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
A novel strategy for analysis of gene homologues and segmental genome duplications
Vladimir N Noskov1, Sun-Hee Leem, Greg Solomon
1Laboratory of Biosystems and Cancer, Center for Cancer Research, National Cancer Institute, NIH, Building 37, Room 5032, Bethesda, MD 20892, USA.
Abstract:
Transformation-associated recombination (TAR) cloning allows selective isolation of a desired chromosomal region or gene from complex genomes. The method exploits a high level of recombination between homologous DNA sequences during transformation in the yeast Saccharomyces cerevisiae. We investigated the effect of nonhomology on the efficiency of gene capture and found that up to 15% DNA divergence did not prevent efficient gene isolation. Such tolerance to DNA divergence greatly expands the potential applications of TAR cloning for comparative genomics. In this study, we were able to use the technique to isolate nonidentical chromosomal duplications and gene homologues.
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