Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Perfectly complementary nucleic acid enzymes.

Scott T Kuhns1, Gerald F Joyce

  • 1Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

Journal of Molecular Evolution
|August 13, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cross-chiral exponential amplification of an RNA enzyme.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

RNA-catalyzed evolution of catalytic RNA.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

Correction to "Cross-Chiral, RNA-Catalyzed Exponential Amplification of RNA".

Journal of the American Chemical Society·2024
Same author

RNA Polymerase Ribozyme That Recognizes the Template-Primer Complex through Tertiary Interactions.

Biochemistry·2023
Same author

Discovery of small molecules that target a tertiary-structured RNA.

Proceedings of the National Academy of Sciences of the United States of America·2022
Same author

Investigation of a failed DNA assay leads to identification of an unexpected contaminant in a commonly used chromatography buffer.

Biotechnology progress·2022
Same journal

Sensing Underwater: Diversifying Selection, Convergent Evolution and Inactivation in Sensory Receptors' Genes of Aquatic Mammals.

Journal of molecular evolution·2026
Same journal

Synonymous Codons as Potential Contributors to Chromatin Stability and Gene Body Methylation in Plants.

Journal of molecular evolution·2026
Same journal

Convergent Functional Genomic Evolution Underlying Repeated Freshwater Colonization in Cetaceans.

Journal of molecular evolution·2026
Same journal

Conditions Enabling the Persistence of Cooperating Synthetase, Ligase, and Mutation-Inhibitor Catalytic Polymers.

Journal of molecular evolution·2026
Same journal

Lineage-Specific Diversification of Nucleoporin Nup98 Genes in Ciliates and Its Evolutionary Implications for the Nuclear Dualism.

Journal of molecular evolution·2026
Same journal

Mitochondrial Genome Evolution: The Influence of Partitioning, Calibration, and Gene Heterogeneity on Pleurodontan Substitution Rates.

Journal of molecular evolution·2026
See all related articles

Researchers engineered complementary nucleic acid enzymes that function from both strands of a DNA duplex. This approach maximizes sequence space utility, enhancing catalytic nucleic acid evolution and function.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Synthetic Biology

Background:

  • Maximizing nucleic acid sequence space is vital for early life and offers advantages in modern organisms.
  • Utilizing both strands of a nucleic acid duplex can increase sequence space density for functional molecule production.

Purpose of the Study:

  • To demonstrate the feasibility of creating functional nucleic acid enzymes from both strands of a duplex.
  • To engineer complementary pairs of nucleic acid enzymes with distinct structures and catalytic activities.

Main Methods:

  • Engineering two pairs of nucleic acid enzymes designed as perfect complements.
  • Assessing the structural and catalytic capabilities of each engineered enzyme.

Main Results:

Related Experiment Videos

  • Both engineered enzymes in each pair adopted distinct structures and catalyzed specific chemical transformations.
  • The activity of each enzyme was comparable to the canonical form of its catalytic motif.

Conclusions:

  • Functional nucleic acids can be generated from both strands of a duplex, effectively doubling sequence utilization.
  • This strategy has significant implications for the evolution of catalytic nucleic acids and optimizing genetic sequence functionality.