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Vitamin E prevents renal dysfunction induced by experimental chronic bile duct ligation
M Clara Ortiz1, Melissa C Manriquez, Karl A Nath
1Department of Physiology and Biophysics, Mayo School of Medicine, Mayo Clinic, Rochester, Minnesota, USA.
Kidney International
|August 13, 2003
Summary
Vitamin E treatment improved kidney function and survival in rats with prolonged cholestasis by reducing oxidative stress and endothelin levels, particularly within the renal system.
Area of Science:
- Nephrology
- Hepatology
- Pharmacology
Background:
- Prolonged cholestasis impairs renal hemodynamics and function.
- Mechanisms may involve oxidative stress, lipid peroxidation products (F2-isoprostanes), and endothelin (ET).
Purpose of the Study:
- To investigate if antioxidant therapy with vitamin E prevents chronic bile duct ligation (CBDL)-induced renal dysfunction.
- To determine if vitamin E reduces F2-isoprostane and ET levels in cholestatic rats.
Main Methods:
- Sprague-Dawley rats received normal or high vitamin E diets before and after CBDL or sham surgery.
- Physiologic studies were conducted 21 days post-surgery.
Main Results:
- CBDL rats exhibited reduced mean arterial pressure (MAP), renal blood flow (RBF), glomerular filtration rate (GFR), and excretory function, with increased renal vascular resistance (RVR).
- Elevated systemic and renal venous F2-isoprostanes and ET were observed in CBDL rats.
- Vitamin E normalized MAP, RBF, GFR, RVR, and excretory function, improving survival. It selectively reduced renal F2-isoprostane and ET levels.
Conclusions:
- Vitamin E administration improves MAP and renal function in cholestatic rats.
- Selective reduction of renal oxidative stress and ET by vitamin E suggests local redox balance is crucial for CBDL-induced renal dysfunction.